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Fig. 4 | BMC Genomics

Fig. 4

From: Genome-wide methylation analysis reveals differentially methylated loci that are associated with an age-dependent increase in bovine fibroblast response to LPS

Fig. 4

Gene Expression post LPS Treatment of Selected Genes from RRBS Analysis in Angus and Holstein Fibroblast Cultures. Expression of six genes more methylated in young cultures, (a) PIK3R1, (b) FES, (c) TNFSF13, (d) NFATC1, (e) TCF7 and (f) RORA were measured in fibroblasts isolated from 19-month Angus and Holstein cows (n = 4 per group) at 0, 2 and 8 h post LPS. Expression was measured by RT-qPCR and values are presented as dCT in comparison to β-actin. Fold change gene expression is displayed in parentheses above each significantly different time point, whereby positive values indicate Holstein > Angus and negative values indicate Angus > Holstein. All values are displayed as mean (+/− SEM). Significance was determined using an unpaired Student’s t Test at each time point post LPS treatment and ** = P < 0.01, * = P < 0.05, † = P < 0.10

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