Genome-wide methylation analysis reveals differentially methylated loci that are associated with an age-dependent increase in bovine fibroblast response to LPS

Table 5 Summary of selected innate response genes hyper-methylated in young cultures

Gene name	Number of differentially methylated sites^a	Location of differentially methylated sites	Expression (Old > Young) at 0, 2 & 8 H post LPS^b	Expression (Holstein > Angus) at 0, 2, & 8 H post LPS^b
FES	2	Promoter, Exon	5.7, 4.3, 6.3	3.7, 5.4, 4.8
NFATc1	3	Promoter, Intron	1.8, 1.8, ---	2.2, 6.5, 1.7
PIK3R1	2	Promoter, Intron	3.0, 2.6, 2.5	---, 2.4, (−1.9)
RORA	5	Intron	---, ---, 2.1	2.0, 2.2, ---
TNFSF13	1	Promoter	2.0, 1.6, 2.8	---, ---, ---
TCF7	2	Promoter	---, ---, ---	4.5, ---, 4.3

^aNumber of differentially methylated CpGs (P < 0.05, >25% meth diff)
^bDisplayed as fold change at 0, 2 or 8 H post LPS. Positive values indicate (Old > Young) or (Holstein > Angus), negative values indicate (Young > Old) or (Angus > Holstein) and --- signifies P > 0.10

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