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Fig. 1 | BMC Genomics

Fig. 1

From: Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocols

Fig. 1

Percentage change in Assigned reads, Multimapped reads and Ambiguous reads. Barplots of the percentage change in reads assigned by featureCounts in the single-end (SE, purple bars) compared to the paired-end strand-specific data (PE) in a total of 24 samples from four experiments (Exp1, Exp2, Exp3, Exp4) and percentage change in non-strand-specific paired-end data (NS, green bars) compared to the paired-end strand-specific data (PE) in a total of 18 samples from three experiments (Exp2, Exp3, Exp4). a Assigned reads, SE comparison, (b) Assigned reads, NS comparison (c) Multimapped reads, SE comparison (d) Multimapped reads, NS comparison (e) Ambiguous reads, SE comparison (f) Ambiguous reads, NS comparison

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