Fig. 1

WSD-induced weight gain and insulin resistance are reversed by CR. a Study design. Animals were maintained in individual housing while consuming chow diet for the first 2 months, followed by Western-style diet (WSD) for 6 months, and caloric restriction (CR) on chow for 4 months. Experimental procedures (DEXA, GTT and muscle biopsies) were performed at the end of each dietary period. Body weight (b), total body fat (c), and lean mass (d) were determined by DEXA, and AUC glucose (e), AUC insulin (f), fasting glucose (g) and fasting insulin (h) were determined by GTT, as described in “Materials and Methods”. HOMA-IR (i) was calculated as described [92]. HbA1c (j) was determined during GTT. Error bars are Means ± SEM, n = 6. *p < 0.05, **p < 0.01 by repeated-measure one-way ANOVA