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Fig. 5 | BMC Genomics

Fig. 5

From: Accurate characterization of the IFITM locus using MiSeq and PacBio sequencing shows genetic variation in Galliformes

Fig. 5

The read alignment views in Artemis showing RNA-Seq data from the different studies. Top panel: the ‘coverage view” showing a separate plot for each BAM mapped to our PacBio contig 2 (40Kb region). The coverage shows only data relative to constitutive expression level of chIFITMs in immune-relevant tissues and cell lines (lung, trachea, spleen, liver, DF1, CEF, HD11, DT40). Bottom panel: the “stack view” (paired reads: blue, single reads and/or reads with an unmapped pair: black; reads spanning the same region: green) to show in more detail read depth across each chIFITM transcript. All the features were annotated manually blasting the sequences from the latest version of the chicken genome. Cyan: CDS region, grey: mRNA, white: gene (overlapping with mRNA features)

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