Table 1 PacBio and Illumina MiSeq de novo assembly and mapping statistics
| Â | PacBio RSII | Illumina MiSeq | ||
|---|---|---|---|---|
Number of reads | 78,140 | 665,450 | ||
Number of Bases | 401,758,407 | 199,635,000 | ||
Mean Read Length | 5141 | 300 | ||
De novo assembly | ||||
Assembly software | HGAP | IVA | ||
Polished contigs | 6 | 13 | ||
Sum of contigs length | 4,818,915Â bp | 277,830Â bp | ||
Largest fragment | 2,323,934 bP | 73,284Â bp | ||
N50a | 1,102,549Â bp | NA | ||
Mapping | ||||
Reference | Mapped reads | Mean coverage | Mapped reads | Mean coverage |
Chr.5 Gallus gallus v4 | 33,892 | 193 | 586,297 | 607 |
Chr.5 Gallus gallus v5 | 34,068 | 196 | 693,474 | 440 |
PacBio_contig N.2 | NA | NA | 606,994 | 599 |