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Fig. 5 | BMC Genomics

Fig. 5

From: Assembly and use of high-density recombinant peptide chips for large-scale ligand screening is a practical alternative to synthetic peptide libraries

Fig. 5

Re-evaluation of chip-derived Streptavidin binders in complementary setups. a In vitro fluorescence polarization assays using constant amounts of 5’FAM labeled (chemically synthesized) peptides plus increasing concentrations of recombinant protein. Data are reported as mean from 3 independently performed measurements. Standard deviation was below 15% for all values. b Binding kinetics (association of pre-determined amounts 5’FAM labeled peptides to Streptavidin biosensors) were measured via biolayer-interferometry. Kd values as calculated from the BLItz Pro software are listed

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BMC Genomics

ISSN: 1471-2164

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