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Fig. 6 | BMC Genomics

Fig. 6

From: Assembly and use of high-density recombinant peptide chips for large-scale ligand screening is a practical alternative to synthetic peptide libraries

Fig. 6

Functional analysis of hit peptides as putative affinity/purification reagents. a Schematic illustration of underlying cloning procedure. b S. aureus clumping factor was C-terminally tagged with either Strep-tag I, Strep-tag II, F14.5 or B5, as indicated, and shuttled together with the original (unmodified) construct in pDEST™17. Recombinant protein expression (total lysate; S: soluble supernatant) and subsequent ClfB (37 kDa) protein purification on magnetic Streptavidin beads (E: eluate) were detected on dot blot as well as Western Blot level (12.5% SDS-PAGE), applying either HRP-labeled Streptavidin/StrepTactin or an α-6xHIS control antibody. Molecular mass standards (M) are indicated on the left

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BMC Genomics

ISSN: 1471-2164

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