TY - JOUR AU - Shagin, Dmitriy A. AU - Turchaninova, Maria A. AU - Shagina, Irina A. AU - Shugay, Mikhail AU - Zaretsky, Andrew R. AU - Zueva, Olga I. AU - Bolotin, Dmitriy A. AU - Lukyanov, Sergey AU - Chudakov, Dmitriy M. PY - 2017 DA - 2017/06/05 TI - Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries JO - BMC Genomics SP - 440 VL - 18 IS - 1 AB - Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3′-end of unwanted amplification primer (NOPE oligo) simultaneously provides a template for its elongation. This elongation disrupts specificity of unwanted primer, preventing its further participation in PCR. The suggested approach allows to rationally manage the course of PCR reactions in order to facilitate analysis of complex DNA mixtures as well as to perform multistage PCR bypassing intermediate purification steps. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-017-3815-2 DO - 10.1186/s12864-017-3815-2 ID - Shagin2017 ER -