Fig. 1From: Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded librariesGeneral principle of NOPE method. NOPE oligo is complementary to the 3′ end of the unwanted primer. Upon binding to the 3′ end of the primer, NOPE oligo provides a template for the primer extension, thereby producing new “nonsense” 3′-end (i.e. the 3′-end that is non-complementary to the amplified template)Back to article page