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Fig. 6 | BMC Genomics

Fig. 6

From: Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

Fig. 6

Comparison of NOPE-R3 and NOPE-R0 oligo performance. Lanes A1-A3 represent reactions containing two primers in the 1st PCR – forward TruSeq-short and reverse EGFR-ex20-R1; lanes B1-B3 represent reactions containing only the reverse primer EGFR-ex20-R1. NTC – non template control. See Methods for details. Note absence of PCR product in B3 reaction that has been amplified for 11 more cycles compared to A3 and 9 more cycles compared to B1

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