Primer | Application | Sequence |
---|---|---|
Primers for linear PCR | ||
EGFR-ex20_NNN | PCR-based introduction of UMI (UMI-containing primer), contains partial sequence of TruSeq Illumina adapter | ACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNCATCTGCCTCACCTCCACCGT |
EGFR- ex18-1reg_NNN | ACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNGAAGCTCCCAACCAAGCTC | |
EGFR- ex19-1reg_NNN | ACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNCTGTCATAGGGACTCTGGAT | |
EGFR -ex21_R_NNN | ACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNTCTTTCTCTTCCGCACCCAG | |
EGFR -ex22_NNN | ACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNCATCCCAAGGTGCCTATCAA | |
NOPE oligos | ||
NOPE-R1 | Neutralization of EGFR-ex20_NNN | GCACGCGTCGACGGTGGAGGTG-BHQ1 |
NOPE-R2 | ACACGACGCTCTTCCGATCTACGGTGGAGGTGAGGCAG-BHQ1 | |
NOPE-R3 | GCACGCGTCGACGGTGGAGGTGAGGCAG-BHQ1 | |
NOPE-R0 | ACGGTGGAGGTGAGGCAG-BHQ1 | |
NOPE-EGFR- ex18 | Neutralization of EGFR-ex18-1reg_NNN | TCACACGTCTGAGCTTGGTTGGGAGCTTC-BHQ1 |
NOPE-EGFR-ex19 | Neutralization of EGFR- ex19-1reg_NNN | ATACTCTTCGATCCAGAGTCCCTATGACAG-BHQ1 |
NOPE- EGFR-ex21 | Neutralization of EGFR-ex21_R_NNN | CTTGACGTCACTGGGTGCGGAAGAGAAAGA-BHQ1 |
NOPE- EGFR-ex22 | Neutralization of EGFR-ex22_NNN | ACTCTCATCCTTGATAGGCACCTTGGGATG-BHQ1 |
Primers for the 1st PCR | ||
EGFR-ex20_R1 | Reverse nested EGFR-ex20-specific primer 1 | TGTTCCCGGACATAGTCCAG |
EGFR-ex18-1reg_ R1 | Reverse nested EGFR-ex18-specific primer 1 | AACGCACCGGAGCCCAGCACT |
EGFR-ex19-1reg_R1 | Reverse nested EGFR-ex19-specific primer 1 | GATTTCCTTGTTGGCTTTCGGA |
EGFR-ex21_F1 | Forward nested EGFR-ex21-specific primer 1 | CTGGTGAAAACACCGCAGCA |
EGFR-ex22_R1 | Reverse nested EGFR-ex22-specific primer 1 | GCTCCAGACATCACTCTGGT |
TruSeq-short | Step-out primer 1 | TACACGACGCTCTTCCGATCT |
Primers for 2nd PCR | ||
EGFR-ex20_R2 | Reverse nested EGFR-ex20-specific primer 2, contains partial sequence of TruSeq Illumina adapter | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTACATAGTCCAGGAGGCAGC |
EGFR-ex18-1reg_R2 Br | Reverse nested EGFR-ex18-specific primer 2, contains partial sequence of TruSeq Illumina adapter | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCGGAGCCCAGCACTTTGATC |
EGFR-ex19-1reg_R2 Br | Reverse nested EGFR-ex19-specific primer 2, contains partial sequence of TruSeq Illumina adapter | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTGTTGGCTTTCGGAGATGTT |
EGFR-ex21_F2 Br | Forward nested EGFR-ex21-specific primer 2, contains partial sequence of TruSeq Illumina adapter | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAAACACCGCAGCATGTCAAG |
EGFR-ex22_R2 Br | Reverse nested EGFR-ex22-specific primer 2, contains partial sequence of TruSeq Illumina adapter | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTACATCACTCTGGTGGGTATAG |
TruSeq-long | Step-out primer 2, contains sequence of TruSeq Illumina adapter | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT |
Primers for 3rd PCR (introducing Illumina indexes) | ||
TruSeq-Index1 | Reverse step-out primer, anneals on the EGFR-ex20_R2, contains complete sequence of TruSeq Illumina adapter with sample index | CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGT |
Illumina-Dir | Forward step-out primer 3 | AATGATACGGCGACCACCGAGATC |
Real time PCR probes | ||
Z-IGFR ex20 | Real time PCR probe for exon 20. | Fam-AGCTCATCACGCAGCTCATGCCCTT-BHQ1 |
Z-TruUni | Universal real time PCR probe for TuSeq adapter | Fam-ACACTCTTTCCCTACACGACGCTCTT-BHQ1 |