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Table 2 NOPE oligo quantities tested during concentration optimization

From: Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

№ of sample 1 2 3 4 5 6 7 8
NOPE-R3/EGFR-ex20_NNN ratio 1:1 2:1 4:1 5:1 8:1 10:1 12:1 15:1
NOPE-R3 oligo concentration, μM 0.1 0.2 0.4 0.5 0.8 1.0 1.2 1.5