From: Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries
â„– of sample
1
2
3
4
5
6
7
8
NOPE-R3/EGFR-ex20_NNN ratio
1:1
2:1
4:1
5:1
8:1
10:1
12:1
15:1
NOPE-R3 oligo concentration, μM
0.1
0.2
0.4
0.5
0.8
1.0
1.2
1.5