Fig. 2

Proteomic responses of oysters to ocean acidification and warming. a A representative gill proteome map of B2 line oysters. Protein spots differentially regulated due to pH and/or temperature treatments are highlighted. Numbers associated with each protein spot correspond to arbitrary identifiers generated by PDQuest during image analysis. Numbers in bold indicate the 23 protein spots that were successfully identified by mass spectrometry. pI, isoelectric point; MW, molecular weight in kDa. b Non-metric multidimensional scaling (NMDS) plot summarising the cumulative normalised intensities of 23 differentially regulated protein spots among B2 oysters exposed to elevated CO₂ and/or elevated temperature (T). Each point represents the spot intensity levels of pooled samples containing 5 oysters each per condition. c Heat map of mean normalised intensities of differentially regulated proteins. Proteins are identified by their spot numbers followed by their putative identifications obtained using mass spectrometry. Protein names and fold differences are detailed in Additional file 2: Table S2. aCO₂ + aT: ambient CO₂ and ambient temperature; aCO₂ + eT: ambient CO₂ and elevated temperature; eCO₂ + aT: elevated CO₂ and ambient temperature; and eCO₂ + eT: elevated CO₂ and elevated temperature