Fig. 5
The results of the functional annotation of significant genes. Enriched gene ontology (GO) biological processes (BP), molecular functions (MF) and cellular components (CC) by significant (FDR < 10−4) protein coding genes from unpaired RNA-seq (edgeR) and HTA (limma) analyses are intersected (a). Only ontology terms with FDR < 0.01 were considered. The complete list is given in Additional file 3 and summarized in Additional file 1: Figure S7. The expression of genes related to cellular component ontology terms uniquely identified by RNA-seq (red lines) or HTA (blue lines) is shown (b-c). The distributions of gene expressions are based on sequencing (b) and microarray (c) data. Both analyses show that genes associated with an ontology uniquely found in RNA-seq analysis have a higher expression than genes with HTA-specific ontology terms (arrows and yellow area)