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Fig. 1 | BMC Genomics

Fig. 1

From: A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

Fig. 1

Generation of sequencing libraries and experimental design. a Schematic representation of the workflow to generate the sequencing libraries. RNA of the SEQC samples A and B is heat degraded to obtain three distinct RNA input qualities. Several input amounts between 1 ng and 100 ng are selected from the degraded RNA in triplicate and used for the library preparation with one of the three protocols (TruSeq, Ribo-Zero, or RNA Access). b Overview of the combinations of degradation stage, input amount, and library preparation protocol considered in this study. A green tick indicates a combination that was sequenced, a blue cross indicates a combination for which we decided not to generate a library either because the input amount was higher than the maximum recommended input amount (for RNA Access) or because previously published studies suggested inferior performance on degraded samples (for TruSeq), and a red cross indicates a combination for which no library was generated because libraries with higher input amounts already performed poorly

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