Table 1 Summary of the assessment of the three protocols for different input amounts and sample degradation stages
From: A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples
RNA seq Protocol | RNA Input | 1Â ng | 2Â ng | 5Â ng | 10Â ng | 20Â ng | 100Â ng | Conclusion |
|---|---|---|---|---|---|---|---|---|
TruSeq stranded mRNA | Intact | = | + | ++ | +++ | +++ | +++ | +Works well at low amounts down to 5Â ng -Captures polyadenylated RNAs only -Not suited for degraded or highly degraded samples |
Degraded | * | * | * | * | * | ++ | ||
Highly deg. | * | * | * | * | * | * | ||
RiboZero stranded RNA | Intact | + | + | ++ | ++ | ++ | ++ | +Works well over all input amounts +Captures all RNAs (coding & non coding) +Compares well to mRNA protocol +Well suited for degraded samples -Requires higher sequencing depth -Not suited for highly degraded samples |
Degraded | + | + | ++ | ++ | ++ | ++ | ||
Highly deg. | * | * | * | −− | − | − | ||
RNA access | Intact | − | = | ++ | ++ | ++ | * | +Performs well on all samples down to 5 ng +Requires less sequencing depth +Suited for degraded and highlydegraded samples -Captures only preselected RNAs and is only available for human samples -Less similar to the other two protocols |
Degraded | − | = | ++ | ++ | ++ | * | ||
Highly deg. | −− | − | = | + | + | * |