Fig. 7
From: Relatively frequent switching of transcription start sites during cerebellar development
Alternative TSSs in contactin associated protein-like 2 (Cntnap2) and experimental validation of its crossover switching events with Real-time PCR. a Schematic DNA structure of Cntnap2, alternative mRNA variants and truncated protein structure of the short isoform. b in situ expression of Cntnap2 in mouse cerebellum at E14.5 (from GenePaint). c HeliscopeCAGE expression data for the two alternative TSSs during cerebellar development. X-axis: time, from embryonic day 11 (E11) to postnatal day 9 (P9). Y-axis: expression level measured in tpm (tags per million). d qRT-PCR expression data demonstrating a crossover TSS switching events between E12 (as well as E15) and P9. X-axis: time at E12, E15 and P9. Y-axis: expression level measured in RQ (relative quantity against H2O as negative control)