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Table 1 Transcriptome assembly (BinPacker CD-hit-est Transrate Corrected) performance metrics for: contig number, TransRate score (Score), BUSCO indices: % single copy orthologs (% SCO), % duplicated copy orthologs (% DCO), % fragmented (% frag), and % missing (% miss), as well as Salmon mapping rates (% mapping) for the optimized testes assembly. Dammit transcriptome assembly annotation statistics, including searches in the program TransDecoder for open reading frames (ORFs) and searches for homologous sequences in five databases: Rfam, Pfam-A, Uniref90, OrthoDB, and BUSCO. Percentages were calculated from the count number of each parameter divided by the total number of contigs in the transcriptome (155,134). The only exception to this calculation is for complete ORFs, which were calculated as a percentage of the total ORFs (75,482). The BUSCO results for the annotated assembly are not shown here as they are identical to those for the un-annotated assembly

From: Characterizing the reproductive transcriptomic correlates of acute dehydration in males in the desert-adapted rodent, Peromyscus eremicus

Transcriptome Assembly Statistics

Contig #

Score

% SCO

% DCO

% frag

% miss

% mapping

155,134

0.335

77

27

5.9

16

92.14

Dammit Annotation Statistics

Search Type

TransDecoder

Rfam

Pfam-A

Uniref90

OrthoDB

Dammit

Parameter

Total ORFs

Complete ORFs

ncRNAs

Protein Domains

Proteins

Orthologs

Total Annotated Contigs

Count

75,482

43,028

937

25,675

62,865

51,806

77,915

Percentage

48.7%

57.0%

0.6%

16.6%

40.5%

33.4%

50.2%