Fig. 7

Functional characterization of McTPS1. a In vitro enzymatic assay of recombinant 6His-McTPS1 protein using either GPP or FPP as substrate. Control, TPS assay of the heat-inactivated 6His-McTPS1 recombinant protein with GPP. The reaction products were analyzed by chiral phase GC-MS. The peak obtained from in vitro assay was identified with the authentic standard, (±)-linalool. Mass spectra of the peak from the assay and (R)-linalool are shown at the bottom of chromatograms. b In vivo characterization of McTPS1 by transient expression of McTPS1 in N. benthamiana leaves. VOCs were collected from McTPS1 or GFP infiltrated N. benthamiana leaves and analyzed by chiral phase GC-MS. c (R)-linalool emission levels in transgenic N. tabacum lines overexpressing McTPS1 (#1 and #2, McTPS1-OX) or wild-type (WT). Volatiles were collected by headspace method and analyzed by chiral phase GC-MS method. The quantity of (R)-linalool was determined by calculating the peak area of internal standard camphor (100 ng/μl). Error bars indicate SD (n = 2). nd, not detectable