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Fig. 4 | BMC Genomics

Fig. 4

From: Systematic discovery of novel eukaryotic transcriptional regulators using sequence homology independent prediction

Fig. 4

Mutants lacking CHIQ1 have smaller organs. a-b, Whole plants (a) or rosette leaves (b), of wild type (Col-0, left or top), chiq1–1 (middle), and chiq1–1 complemented with CHIQ1 (B12, right or bottom) grown in soil for 7 weeks. Leaves are ordered from the oldest (left) to the youngest (right). c Height of the primary inflorescence stem in wild type (black), chiq1–1 (white), and complemented (gray) plants grown in soil for 11 weeks. Stature of chiq1–1 plants is reduced by 53% compared to the wild type and 42% compared to the complemented line (* = p-value: 2E-34 against wild type and 2E-25 against complemented line, t-test). n = 30 per genotype from 8 independent experiments. d Measurements of leaf area from wild type (black), chiq1–1 (white), and complemented (gray) plants grown in soil for 7 weeks. n = 8 per genotype from 3 independent experiments. c-d Error bars represent standard error from 3 independent experiments. e-g Expression of the CHIQ1-GUS transgene driven by CHIQ1 promoter in the root apical meristem (e), shoot apical meristem and leaf primordia of 2 day-old seedlings (f) and rosette of 14 day-old plants (g) grown on MS media. Each image is a representative of at least three independent experiments with n = 10 plants. At least three independent transgenic lines were analyzed

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