Table 5 Expression profiles comparison between NGS transcriptome profiling and NanoString nCounter^® Gene Expression Assay

Gene

Pathway involved

AFM

AFC

log2

AFM

AFC

log2

ANXA7

Gland morphology

1.89

35.49

4.23

951.94

1990.60

2.09

PRMT5

Amino phosphate metabolism

3.41

103.85

4.92

174.59

162.56

−1.07

HK1

Amino sugar metabolism

59,911.80

11,492.50

−2.38

724.05

926.69

1.28

AMC

AFC

log2

AMC

AFC

log2

MGAT

N-glycan biosynthesis

20.27

256.55

3.66

1337.15

2571.24

1.92

HADHB

Fatty acid synthesis

7.72

53.47

2.79

2300.80

2619.53

1.14

ARF1

Vesicle biogenesis

17.24

73.10

2.08

5540.25

7430.06

1.34

RAB5

0.42

35.24

6.38

2276.53

3340.04

1.47

AA

EBN producing swiftlet

log2

AA

EBN producing swiftlet

log2

CBPD

Vesicle biogenesis

8.93

63.31

2.82

10,631.67

11,824.94

1.11

MGT

20.27

256.55

3.66

1337.15

2571.24

1.92

PDHB

Alanine and aspartate metabolism

894.02

135.64

−2.72

1709.22

2106.15

1.23

ARF4

EGFR signalling pathway

0.68

48.95

6.16

3188.13

6970.01

2.19

BCAR1

6.40

51.83

3.01

275.97

777.42

2.82

CAV1

0.69

50.20

6.17

3476.29

5640.91

1.62

CEBPA

8.62

49.82

2.52

1874.12

2127.62

1.14

ELF3

6.81

603.60

6.46

2553.18

2836.8

1.11

EPS15

0.19

10.03

5.65

1242.43

1915.74

1.54

EPS8

0.75

13.66

4.17

1921.08

2849.26

1.48

JUN

0.41

11.50

4.80

2756.44

2784.66

1.01

REPS1

6.79

21.52

1.66

1148.45

1440.29

1.25

STAT3

2.45

53.38

4.44

985.67

1713.33

1.74

VAV2

49.55

239.91

2.27

68.78

554.00

8.06

ZPR1

1.07

13.24

3.61

1217.42

2078.00

1.71

ACAD

Fatty acid synthesis

1.52

25.48

4.05

1778.95

2282.97

1.28

ALG2

N-glycan biosynthesis

6.54

90.86

3.79

952.67

1376.55

1.44

MGAT1

4.61

91.06

4.30

66.28

1089.17

16.43

RPN1

66.40

552.36

3.05

6806.71

9113.06

1.34

FUT8

13.17

84.07

2.67

467.63

2465.45

5.27

RPN2

1.06

71.02

6.05

2570.82

3420.57

1.33

G6PD

Glutathione synthesis

0.18

46.66

8.00

85.54

209.11

2.44

RARB

Vitamin A and carotenoid synthesis

11.84

49.92

2.07

1182.35

1190.05

1.01

RARG

8.23

296.86

5.17

527.79

1050.6

1.99

RBP2

12.19

45.13

1.88

943.37

1745.05

1.85

RDH10

0.86

10.44

3.59

4869.85

7627.12

1.57

RDH12

37.25

100.33

1.42

280.34

790.51

2.82

ILVBL

4.42

87.32

4.30

5.93

347.25

58.55