Fig. 10
From: Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing
qRT-PCR validation of drought-responsive miRNAs (a) and genes (b) in tomato. The 2-ΔΔCt was used to calculate the fold change of expression in qRT-PCR analysis, with U6 and Actin 4 as reference for miRNA and genes respectively. All experiments were repeated three times, and the expression data were log2 transformed before analysis. Error bars represent the standard deviation of the replicates. The deep sequencing results with log2 fold change are shown here to compare with qRT-PCR results. S: a drought-sensitive cultivar, M82, T: a drought-tolerant introgression line, IL9–1