Fig. 4

Tissue and RNA-seq library preparation effects on lncRNA detection and expression. a There is a positive relationship between the number of annotated genes and candidate lncRNA detected in each tissue; the pie charts represent the cumulative TPM of that tissue with the turquoise correlated to the expression of the protein-coding transcripts and red to the candidate lncRNA expression. The pies outlined in yellow were rRNA-depleted RNA-seq libraries, pies outlined in black were Ovation RNA-seq libraries and the pies outlined in blue were the polyA-captured RNA-seq libraries. b The hierarchically clustered heatmap also shows clustering on a tissue and RNA-seq library level. c There is a distinguishable difference in the number on lncRNA that seem to be unique to a given tissue, with the skin having the largest number of unique lncRNA and the highest cumulative expression associated with its unique lncRNA. The green line represents the cumulative TPM of all the uniquely present lncRNA, divided by 5 for scaling