Fig. 4

Single-cell RNA-seq profiling of cultured human and mouse cell lines and mouse pancreatic islets. a Unsupervised principal component analysis (PCA) for human cell lines based on the 500 most variable genes. b Unsupervised PCA for mouse cell lines based on the 500 most variable genes. c Hierarchical clustering of mouse pancreatic islet cells based on known cell type markers. Four subpopulations were identified based on four clusters indicated in the hierarchical clustering dendrogram. Cell type labels (alpha, beta, delta and PP) were assigned based on the expression of known marker genes