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Fig. 9 | BMC Genomics

Fig. 9

From: Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

Fig. 9

Evaluation of the new ssRNA-seq pipeline using RNA from tumor samples. 100 ng total RNA from 12 different tumor samples was used as input to generate libraries using the new protocol. Adapter-ligated libraries were enriched with 13 cycles of PCR. a Library yield. b Correlation of expression. Pearson’s correlation coefficient was calculated pair-wise showing higher correlation between the new lower input libraries and their previous higher input counterparts

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