Skip to main content

Table 5 Essential reactions unique to resistant parasites

From: Novel Plasmodium falciparum metabolic network reconstruction identifies shifts associated with clinical antimalarial resistance

Reaction Reaction Formula EC Number Reaction Function Genes
CO2tmt CO2[m] < => CO2[c] - CO2 transport -
EX_folate4 p-aminobenzoate[e] < => - p-aminobenzoate exchange -
EX_fru(e) fructose[e] < => - fructose exchange -
EX_thm(e) thiamine[e] < => - thiamine exchange -
FRUt1r fructose[e] < => fructose[c] - fructose transport PFB0210c
FUM_mt fumarate [m] + H2O[m] < => malate[m] 4.2.1.2 fumarate hydratase in the TCA cycle PFI1340w
FUMtmt fumarate[m] < => fumarate [c] - fumarate transport into mitochondria -
GHMT2r serine[c] + thf[c] < => glyine[c] + H2O[c] + mthf[c] 2.1.2.1 serine hydroxymethyltransferase in folate synthesis PFL1720w
GLYCL_mt glycine[m] + NAD[m] + thf[m] < => CO2[m] + mlthf[m] + NADH[m] + NH4[m] many glycine cleavage system in folate synthesis and amino acid metabolism PF13_0345a
PFL1550w
MAL13P1.390
PF14_0497
PF11_0339
GLYtmt glycine[m] < => glycine[c] - glycine transport into mitochondria -
HEX7 ATP[c] + fructose[c] = > ADP[c] + fructose-6-phosphate[c] + h[c] 2.7.1.1 hexokinase of glycolysis PFF1155w
MDHm malate[m] + NAD[m] < => h[m] + NADH[m] + oxaloacetate[m] 1.1.1.37 malate dehydrogenase in the TCA Cycle PFF0895w
MLTHFtmt mthf[m] < => mthf[c] - mthf transport into mitochondria MAL8P1_13*
PF11_0172
NADPHtmt NADPH[c] < => NADPH[m] - NADPH transport into mitochondria -
NADPtmt NADPc] < => NADP[m] - NADP transport into mitochondria -
NH4tmt NH4[m] < => NH4[c] - NH4 transport into mitochondria -
OAAtmt oxaloacetate [m] < => oxaloacetate[c] - oxaloacetate into mitochondria -
THFtmt thf[m] < => thf[c] - thf into mitochondria -
THMt3 h[c] + thiamine[e] < => h[e] + thiamine[c] - thiamine import -
TMDPK ATP[c] + thiamine[c] = > AMP[c] + h[c] + thiamine diphosphate[c] 2.7.6.2 thiamine diphosphokinase in cofactor metabolism PFI1195c
pABAt p-aminobenzoate[e] < => p-aminobenzoate[c] - p-aminobenzoate import MAL8P1_13a PF11_0172
  1. All reactions in table are predicted to be lethal when removed from both Cambodia and Vietnam resistant models
  2. a, deleted from at least one resistant model due to expression constraints by MADE localization: [e] extracellular, [c] cytoplasmic, [m] mitochondria, [ap] apicoplast