Table 2 Information of the primers used in this study
Loci | Primer senquence | Size | Primer Efficiency | |
|---|---|---|---|---|
Forward (5′-3′) | Reverse (5′-3′) | (bp) | (mean ± SE) | |
TCONS_00311264 | TGTGGAGGAAACGAGGGT | GAAAGATGCCGCTTGGA | 224 | 0.95 ± 0.01 |
TCONS_00392681 | TATTCTGGGCAGGATTG | GCAGGTCTTTCGAGGTA | 160 | 0.97 ± 0.04 |
TCONS_01199263 | CTCGCTCAACTCAGCATTC | TGGACAAGGGTTAGAAGTAAAT | 228 | 0.84 ± 0.04 |
TCONS_01743394 | TATTGAAATGCGTGAGGCT | CTGGAACGAGCAAGGAGA | 187 | 0.88 ± 0.02 |
TCONS_00465264 | GCTGCGCTCTATTCTGTG | GTATGCCAGGAGGGAGGA | 121 | 0.86 ± 0.02 |
TCONS_00625772 | GGCTTCCGCCTCGTTGAT | CTGGCTGCCTTCGTTTCC | 122 | 0.94 ± 0.06 |
TCONS_00869992 | AGGGAGAAGGAGTGAGACAAG | GGGAAGGCAGATGGACAA | 174 | 0.92 ± 0.03 |
TCONS_01520466 | TTTCTTGGGTAAACATCTGG | GTGACCTTGTTGGGCAGT | 158 | 0.87 ± 0.05 |
NR2F2 | GGACCACATACGGATCTTC | CTGGCTCCTAACGTACTCTT | 190 | 0.94 ± 0.04 |
ENSMUSG00000100005 | GATTAGAGTATCGCATCAGC | CGTGGGTCAGCAAGGTTA | 296 | 0.95 ± 0.02 |