Table 6 Selection of genes which showed upregulated expression in MT-treated testes (MT) compared to ovaries (CO). 40CO, 40 dpf control ovaries. 40MT, 40 dpf MT-treated testes. 60CO, 60 dpf control ovaries. 60MT, 60 dpf MT-treated testes
From: Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development
No. | Gene name | Gene ID | Fold change for control gonads | |
|---|---|---|---|---|
Testis-Enriched Genes | 40MT/40CO | 60MT/60CO | ||
Male sex determination | ||||
1 | amh | 66.56 | 74.09 | |
2 | dmrt1 | NM_205628 | 10 | 27.38 |
3 | gsdf | NM_001114668 | 115.21 | 120.57 |
Sertoli cell differentiation | ||||
4 | gata4 | n.s. | n.s. | |
5 | sox9a | n.s. | n.s. | |
Leydig cell differentiation | ||||
6 | pdgfra | n.s. | n.s. | |
Steroidogenesis | ||||
7 | ar | n.s. | n.s. | |
8 | cyp11c1 | 8.8 | 43.34 | |
9 | cyp17a1 | n.s. | n.s. | |
10 | esr2b | 7.25 | 7.33 | |
11 | hsd11b2 | 7.40 | 16.49 | |
12 | nr5a1a | n.s. | n.s. | |
13 | star | 22.17 | 83.7 | |
GABA and dopamine signalling | ||||
14 | gabrr2b | XM_692394.7 | 12.43 | 25.29 |
15 | drd2l | NM_197935.1 | n.s. | 2.69 |
Spermiogenesis | ||||
16 | klhl10a | XM_002665116 | 29.96 | 31.04 |
17 | odf3b | 14.52 | 34.00 | |
18 | tekt1 | NM_001007397 | 296.47 | 385.53 |
Septin signalling | ||||
19 | sept3 | NM_001024418 | 5.89 | 6.57 |
20 | sept8b | NM_001083566 | 11.92 | 11.60 |
Histone variants | ||||
21 | h1f0 | NM_199552.1 | 322.24 | 493.16 |
22 | h1fx | NM_199276.1 | 18.18 | 32.26 |
23 | h2afy2 | NM_001025502.1 | 12.11 | 26.18 |
24 | histh1l | NM_001017660.2 | 9.44 | 12.75 |
25 | hist2h2l | NM_200117.1 | 5.24 | 7.68 |
Immune response | ||||
26 | mif | NM_001043321.1 | n.s. | 2.15 |
27 | il1b | NM_212844.2 | n.s. | 5.03 |
28 | irf9 | NM_205710.2 | n.s. | 5.51 |
Others | ||||
29 | dkk3b | NM_001089545 | 4.89 | 4.91 |
30 | tp53 | n.s. | n.s. | |