Table 7 Selection of genes which showed downregulated expression in MT-treated testes (MT) compared to ovaries (CO). 40CO, 40 dpf control ovaries. 40MT, 40 dpf MT-treated testes. 60CO, 60 dpf control ovaries. 60MT, 60 dpf MT-treated testes
From: Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development
No. | Gene name | Gene ID | Fold change for control gonads | |
|---|---|---|---|---|
Ovary-enriched genes | 40MT/40CO | 60MT/60CO | ||
Female sex determination | ||||
1 | foxL2a | NM_001045252 | n.s. | n.s. |
2 | foxL2b | NP_001304690 | n.s. | n.s. |
Folliculogenesis | ||||
3 | bmp15 | NM_001020484 | −8.35 | −26.10 |
4 | figla | NM_198919 | −7.76 | −20.07 |
5 | gdf9 | NM_001012383 | −7.13 | −18.24 |
6 | lhx8a | NM_001003980 | −3.74 | −18.32 |
Zona pellucida proteins | ||||
7 | zp2.1 | BC124100 | −5.71 | −33.66 |
8 | zp3b | NM_131696 | −6.27 | −37.89 |
Steroid hormone | ||||
9 | cyp11a1 | NM_152953 | −4.68 | −15.93 |
10 | cyp19a1a | NM_131154 | n.s. | n.s. |
11 | esr2a | NM_180966 | −3.00 | −3.57 |
Vitellogenesis | ||||
12 | vtg1 | NM_001044897.3 | n.s. | n.s. |
Granulosa cell function | ||||
113 | sox11b | NM_131337 | −5.33 | −12.80 |
Wnt signalling | ||||
14 | ctnnb1 | NM_131059 | n.s | n.s |
15 | ctnnbip1 | NM_131594 | n.s | −2.09 |
16 | lef1 | NM_131426 | −3.01 | −6.31 |
17 | tcf7 | NM_001012389 | n.s | n.s |
18 | rspo1 | NM_001002352 | n.s | n.s |
19 | wnt4a | NM_001040387 | n.s | n.s |
20 | wnt11 | NM_001144804 | n.s | −2.26 |
Histone variants | ||||
21 | h1 m | NM_183071.2 | −5.40 | −43.53 |
22 | h2afx | NM_201073.1 | −4.04 | −7.85 |
Histone modification and DNA methylation | ||||
23 | ehmt1a | NM_001030131.2 | −4.58 | −7.73 |
24 | ehmt2 | NM_001113615.1 | −2.81 | −2.49 |
25 | dnmt1 | NM_131189.2 | −4.32 | −6.46 |