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Table 1 Assembly statistics and computational requirements for assembly of the Tara Oceans metagenome. Time required is given in seconds, minutes and hours for illustrative purposes and memory in GB of RAM required

From: Assembling metagenomes, one community at a time

  Tara Ocean
CLC IDBA-UD MEGAHIT metaSPAdes MetaVelvet Omega Ray Meta SPAdes Velvet
Number of contigs (≥ 500 bp) 50,716 163,815 216,938 185,419 67,161 15,982 6128 220,178 57,816
Total length 46,069,409 179,686,756 210,621,485 202,770,058 55,972,515 34,861,819 7,277,214 275,920,632 45,425,460
No. of long contigs (≥ 1 kbp) 10,720 50,498 56,243 48,640 12,590 13,305 2179 70,711 8802
No. of ultra-long contigs (≥ 50 kbp) 0 2 1 37 0 9 0 54 0
Largest contig 39,748 101,400 62,649 141,519 30,177 102,255 41,443 197,381 21,980
N50 880 1166 982 1124 805 2691 1329 1415 749
L50 14,113 38,236 58,246 39,033 21,544 2737 1345 39,617 19,631
Mapping rate (%) 38.98 52.24 55.92 64.03 4117 13.64 8.25 64.46 48.19
Time (seconds) 3527 69,782 10,455 125,862 2527 168,213 16,419 80,039 2342
Time (minutes) 58.78 1163.03 174.25 2097.70 42.12 2803.55 273.65 1333.98 39.03
Time (hours) 0.98 19.38 2.90 34.96 0.70 46.73 4.56 22.23 0.65
Memory required (GB) 16.23 42.84 10.58 66.53 109.37 30.7 42 157.75 109.37