Fig. 3

Large plasmids from parental and derived strains. A protocol adapted from Wheatcroft [53] was used to visualize large plasmids by agarose gel electrophoresis. a Some daughter strain profiles were compared to the parental strains. A449 displays a single band, corresponding to plasmids pAsa5 and pAsa4; 01-B526 displays pAsa5 and pAsa9 bands; and 01-B516 displays a single band, pAsa5. The 01-B526-R2 pAsa5 band is lower than 01-B526 one, since it underwent an ISAS11B-C recombination. 01-B516–11 displays a ZA-rearranged pAsa5 band as well as a pAsa9 one, whereas its parent does not have the pAsa9 plasmid. It was confirmed that all 01-B516-derived strains were mislabelled in the previous studies, and are in fact 01-B526-derived. b 01-B526-R19, the only derived strain whose deletion profile on pAsa5 cannot be explained by IS recombination, was also put on a gel beside Paracoccus aminophilus JCM 7686 [53]. The 01-B526-R19 single band has a molecular weight corresponding to its ZA-rearranged pAsa5 and pAsa9 fused together