Fig. 6

Histone acetylation in ethylene regulated genes in ein2-5. a Boxplot showing the peak breadths at their associated- ethylene regulated genes in ein2-5 under air and ethylene treatment. “U” indicates peak associated-ethylene up regulated genes, n = 405; “D” indicates peak associated-ethylene down regulated genes, n = 634. The ** indicates P < 0.001 by t-test. b Boxplot showing the histone marks enrichment (RPKM) in 1000 bp around TSS at their associated- ethylene regulated genes in ein2-5. “U” indicates peak associated-ethylene up regulated genes, n = 405; “D” indicates peak associated-ethylene down regulated genes, n = 634. The ** indicates P < 0.001 by t-test. c-e Heat maps showing c H3K9Ac, d H3K14Ac, and e H3K23Ac differential enrichment (cutoff P ≤ 0.05) on genes differentially expressed in response to ethylene in Col-0 and ein2-5 mutant seedlings. Only the genes marked by H3K9Ac, H3K14Ac, and H3K23Ac in Col-0 plants were used for this heat map. Each line in the heat map represents the representative histone-marked genes, first two lanes of the heat maps in c-e are the normalized M value (obtained using MAnorm, M = log2 (Read density in C₂H₄/Read density in air)) for differential peaks, and the last lanes of the heat maps in c-e are the fold changes of gene expression