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Fig. 1 | BMC Genomics

Fig. 1

From: Common and phylogenetically widespread coding for peptides by bacterial small RNAs

Fig. 1

Most sRNAs have at least one potential protein-coding ORF. a Top: Length distribution of B. subtilis 168 ORFs annotated in Genbank (orange) compared to those between 10 and 50 amino acids of length in annotated sRNAs (blue) or those arising by chance in shuffled sRNAs (green). 95% confidence limits based on one thousand shuffles of sRNA sequence are shown in grey. Bottom: The same for Clostridium acetobutylicum ATCC 824 ORFs, which have a sharper drop-off around 50 amino acids. b Number of annotated sRNAs and sRNAs with at least one ORF for 14 species, representing 4 phyla. Phyla are represented as colors on the left, and colors on the right indicate when multiple taxonomic classes are represented within one phylum. As in (a), shuffled sRNA sequences generate a number of ORFs comparable in length to the observed amount. Inset: For each species, the percentage of sRNAs having at least one ORF of between 10 and 50 amino acids in length. Box represents median and first and third quartiles, and whiskers extend to the most extreme values. Most species have ORFs in more than 50% of sRNAs

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