Fig. 5
From: Common and phylogenetically widespread coding for peptides by bacterial small RNAs
Mass spectrometry confirms sRNA ORFs in Helicobacter pylori. a Two sRNAs are shown in their genomic context, with short ORFs merged for in-frame overlaps and plotted in a separate track. Both sRNAs had a hit in a mass spectrometry search at a false discovery rate of 0.05 (the middle ORF in the case of shpy997.1). b Validations by synthetic peptide of the peptide/MS matches. On top the spectra from the full shotgun MS/MS experiment are plotted for the hit corresponding to each sRNA ORF. On bottom, the spectra are confirmed with the expected peptide synthesized and run alone on the mass spectrometer