Fig. 6

sRNA coding ORFs are enriched for predicted functions including type I toxins. a B. subtilis 168 sRNAs containing at least one predicted coding ORF were compared to sRNAs with only noncoding ORFs or mock ORFs. Four growth conditions were enriched for high expression of predicted sRNA coding ORFs (1.5-fold increase in coding ORFs over both controls, minimum 5 sRNA coding ORFs). b For all predicted coding ORFs in all species (q≤0.25, n=120), the number of ORFs with at least one predicted transmembrane helix is compared to the number expected by chance based on their shuffled amino acid sequences, sRNA ORFs with q≥0.25, all intergenic ORFs, or size-matched subsets of annotated ORFs. Error bars represent 95% confidence intervals. c As in (b), except that the number of ORFs predicted as type I toxins by the physicochemical classifier are plotted. d Degradation levels of S. aureus ORFs as measured by sequencing of short RNA fragments are plotted. ORFs subject to degradation of double-stranded RNAs have higher levels in wild-type bacteria (y-axis) than in RNAse III mutant bacteria (x-axis). Known and predicted type I toxins are highlighted (blue and red, respectively)