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Fig. 1 | BMC Genomics

Fig. 1

From: Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire

Fig. 1

Overview of the RenSeq protocol: Genomic DNA is sheared to the desired insert size. PCR adapters are ligated to the genomic DNA shear and the shear is amplified. Biotinylated custom made baits are hybridised to the sequences of interest. Molecules with hybridised baits can be separated by Streptavidin magnetic bead capture. The captured DNA sequences are subsequently amplified and the amplified products processed to a MinION library or a PacBio library

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BMC Genomics

ISSN: 1471-2164

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