Fig. 3From: Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoireMinION 2D read assembly pipeline: Basecalling is performed using Metrichor. The FASTA or FASTQ information is extracted from the fast5 files. PCR adapters are removed using cutadapt and chimeric reads are filtered out of the dataset using BLASR. The adapter curated reads are corrected and trimmed in the Canu assembly pipeline and assembled with Canu. After assembly the contigs are polished with nanopolishBack to article page