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Table 7 AUGUSTUS protein prediction results: BLASTP comparison of R-proteins predicted of all assemblies with the 641 predicted R-proteins of the 649 NLR gene reference (amino acids is abbreviated with aa)

From: Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire

 

Canu MinION (nanopolish)

Canu MinION (nanopolish, pilon)

Canu PacBio

HGAP

Geneious

Predicted R-proteins

649

675

611

805

702

NLR-Parser complete

174

283

251

310

380

NLR-Parser partial

475

392

361

495

322

R-proteins with BLASTP hit

368

496

445

533

587

% Database coverage

57.41%

77.37%

69.42%

83.15%

91.57%

% identity to reference

90.67% ± 9.06%

95.53% ± 8.42%

98.19% ± 5.59%

99.22% ± 2.98%

99.87% ± 1.12%

BLASTP alignment length of R-proteins

826aa ± 278aa

921aa ± 301aa

914aa ± 296aa

954 aa ±299 aa

971 aa ±330 aa

BLASTP % alignment length of R-proteins

84.11% ± 18.18%

93.65% ± 13.74%

93.81% ± 13.62%

95.50% ± 10.82%

98.30% ± 6.12%