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Table 4 Subfamily-specific conservation in FNT channelsa

From: Anion-selective Formate/nitrite transporters: taxonomic distribution, phylogenetic analysis and subfamily-specific conservation pattern in prokaryotes

Residueb FocA FdhC NirC-α NirC-β NirC-γ HSC YfdC-α YfdC-β
F202c (TM5a) F (100) F (99.6) F (100) F (100) F (100) F (100) V, I, M (99.7) I, M, L, V (100)
A212c (TM5b) A (100) A (93.4) V (100) A (100) A (82.7) A (100) V (71.5) V, I, L (73.5)
L79d (TM2a) L, V, I (99) L, I (93.4) F, Y (98.1) L (100) L (99.4) L, V, I (96.0) F, Y (100) F (73.5)
F90e (Ω-loop) F (100) V, L, I (86.3) V, L (90.9) F, Y (100) --- F (91.7) F, Y (99.7) F (100)
N172e (TM4) N (100) N (99.6) N (100) N (100) N (99.4) N (96.4) G (99.7) G (99.0)
G86 (Ω-loop) G (99.3) G (100) G (91.9) G (100) N, Q (76) G, S (95.6) R, H, D (81.2) R, H, K (75.5)
S92 (Ω-loop) S (100) G, S (100) G (99.0) G (100) S, G (100) G, S, A (100) E (100) E (90.2)
T93 (Ω-loop) T, S (100) N (91.3) V (84.8) H (98.7) N (95.5) N (88.9) N (93.1) ---
K100 (TM2b) K, R (79.9) A, S, G (99.6) A, S, G (99.5) G, A (100) G, A, S (100) G, S, A, T (97.2) P (75.1) ---
E208 (S-loop) E (100) Q (83) E (100) E (100) E (99.4) E, D (96.8) A, S, T (75.6) ---
T34 (TM1) T, A, S (94.3) L, M, I, V (78.8) --- F (100) Y, F (98.9) F, Y (98.8) L, I (93.9) L, I, V, M (94.1)
L37 (TM1) L, M (81.2) L (83.0) R, K (95.2) G, S, A (96.2) R, K (98.9) --- S, A (96.4) T, S (97.1)
N113 (TM3) N (77.2) N (90.9) N (74.3) V, I (96.2) V, L, I, M (99.4) --- L, V, I, M (85.6) L, I, V, (99.0)
L167 (TM4) L (100) S (75.1) K, R (94.8) K, R (100) R, K, E, D (98.3) R, K (95.6) --- ---
N254 (TM6) N (100) N (97.9) N (100) N (100) N, Q (72.6) N (89.3) F, Y (96.1) F, W (74.5)
  1. aPercentage conservation residues for each subfamily is given in brackets
  2. bResidue numbers correspond to that of formate channel structure as available in the PDB ID: 3KCU. Occurrence of these residues in the transmembrane segments or the functionally important loop regions is also indicated
  3. cPart of the central constriction site; dPart of the cytoplasmic slit in the closed state; ePart of the cytoplasmic slit in the open state
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