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Fig. 3 | BMC Genomics

Fig. 3

From: Impact of blood collection and processing on peripheral blood gene expression profiling in type 1 diabetes

Fig. 3

Sample processing-dependent changes in PBC gene expression. Sample processing-dependent genes were highly enriched for lymphocyte-signature genes (a), ribosomal protein genes (b), and genes that have previously been reported to change in the peripheral blood of at-risk or T1D patients (d, [3, 4]). The majority of housekeeping genes were not affected by sample processing (c). The Venn-diagram (d) shows that ~29% (26 out of 90 on the microarray) of genes shown by Reynier et al. to be differentially expressed in at-risk AA+ first-degree relatives of T1D patients and T1D patients at clinical onset, compared to healthy controls and 12% (54 out of 460 on the microarray) of genes found by Kallionpaa et al. that are changed in the whole blood of T1D progressors compared to healthy controls overlap with the 901 sample-processing dependent genes in our study [3, 4]. Genes highlighted in white in panel d were further validated by QPCR and/or NanoString arrays (see Fig. 3)

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