Table 2 Primer sequences for the KASP assays of the GBS markers linked to the Pis1 gene
Markers | Allele-specific 1 (FAM) | Allele-specific 2 (HEX) | Common |
|---|---|---|---|
KM69 | AACAAAAGCGGGTCCTCTCCG | GAACAAAAGCGGGTCCTCTCCA | CGTGCTGCTTCCTTCCAAGCCAT |
KM70 | ATCGCCAGATGCCACGCACAA | CGCCAGATGCCACGCACAG | AGGGACGTGATCAAATTTTCTTGACGATT |
KM71 | TTTGGACATTATTGGGCTTTATTATACAC | GTTTTGGACATTATTGGGCTTTATTATACAA | CTGGGTTAATAGGTTAGTCCCAAAAGTAA |
KM75 | GGTGCTCGCCCTAAACAATCACA | GTGCTCGCCCTAAACAATCACC | CAGTCCACGTGTCTTTTCTGTAAAACATT |
KM76 | GGCTGGCAGGCTCGCTCG | GGCTGGCAGGCTCGCTCT | TCCGTTGCAGCCGCCAAAGCAA |