Fig. 6

Validation of RNA-Seq results by qRT-PCR. Eight differentially expressed genes (four from the host and four from the fungus) were examined by qRT-PCR at the same time points analyzed by the RNA-Seq. a Tomato genes of healthy vs. C. gloeosporioides-colonized WT tissue. Cytochrome P₄₅₀ and phenylalanine ammonia lyase (pal) were downregulated while lipoxygenase (lox) and glutamate synthase were upregulated. Values were normalized to the values obtained from healthy tissue. b Fungal genes from the C. gloeosporioides colonizing WT vs. RNAi–SlPH tissue. pacC showed no differential effect, NAD⁺-specific glutamate dehydrogenase (gdh2) and ammonia transporter (amet) were downregulated during infection of the RNAi–SlPH line, and pectate lyase B (pelB) was upregulated during infection of the RNAi–SlPH line. Values were normalized to those obtained with the infected WT line. Primer list is provided in Additional file 1