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Table 1 Predicted secondary metabolite loci in Z. tritici

From: In silico prediction and characterization of secondary metabolite biosynthetic gene clusters in the wheat pathogen Zymoseptoria tritici

Cluster number Secondary metabolite class Predicted key biosynthetic gene(s) Predicted transporter Resident transcription factor Subtelomeric Evidence of transcriptional co-expression? AntiSMASH SMURF
Cluster boundary No. of genes Cluster boundary No. of genes
1 NRPS-Like Mycgr3G107072 YES NO NO NO 1:2,172,553:2,212,738 12 1:2,189,682:2,216,244 8
2 Transporter Na YES NO NO NO 1:2,306,586:2,310,597 2 Na Na
3 Terpene Mycgr3G34236 NO NO NO YES 1:2,366,583:2,438,578 28 Na Na
4 Terpene Mycgr3G33174 YES NO NO NO 1:3,518,416:3,535,837 9 Na Na
5 PKS Mycgr3G83965 NO NO NO NO 1:3,821,874:3,860,702 14 Na Na
6 NRPS Mycgr3G16590 YES YES NO NO 1:5,128,643:5,192,491 23 Na Na
7 PKS Mycgr3G67477 YES YES NO YES 1:5,513,274:5,566,181 17 1:5,524,934:5,578,932 20
8 PKS Mycgr3G9788 YES YES YES YES 2:31,258:149,728 37 2:123,001:157,676 11
9 NRPS Mycgr3G19958 NO NO YES NO 2:222,377:310,014 19 2:260,654:284,183 5
10 NRPS Mycgr3G36951 YES NO NO NO 2:420,337:471,819 14 2:433,742:453,243 2
11 NRPS Mycgr3G90558 YES NO NO NO 2:1,008,626:1,058,615 13 2:1,026,842:1,052,480 6
12 Terpene Mycgr3G99148 NO YES NO YES 2:1,934,751:2,046,004 31 Na Na
13 PKS-Like Mycgr3G39149 YES NO YES YES Na Na 3:3,181,477:3,213,160 13
14 NRPS Mycgr3G39931, Mycgr3G40534 YES NO YES NO 4:12,877:43,949 7 Na Na
15 PKS Mycgr3G100089 YES YES YES YES 5:85,463:149,465 22 5:94,206:129,292 12
16 NRPS-Like Mycgr3G93235, Mycgr3G100227 NO YES NO YES 5:1,291,545:1,380,182 31 5:1,309,417:1,324,422 4
17 PKS Mycgr3G72709 YES YES YES YES 6:72,339:170,251 36 6:106,974:121,853 4
18 NRPS Mycgr3G72768 YES NO YES YES 6:283,399:352,537 24 6:310,922:346,260 11
19 NRPS Mycgr3G44313 NO NO NO YES 6:1,808,394:1,845,239 16 6:1,821,703:1,841,259 9
20 NRPS Mycgr3G109989 YES YES NO NO 6:2,243,873:2,278,779 9 Na Na
21 PKS Mycgr3G45348 YES NO YES NO 7:2,566,479:2,608,068 12 7:2,577,096:2,606,813 8
22 NRPS Mycgr3G110642 YES NO YES NO 8:2,346,378:2,374,211 8 8:2,352,913:2,362,665 2
23 NRPS-Like Mycgr3G75370 YES YES NO YES 9:483,924:714,190 65 Na Na
24 PKS Mycgr3G47832 YES YES NO NO 9:1,474,679:1,516,611 12 9:1,494,054:1,506,383 3
25 Terpene Mycgr3G76129 YES YES NO NO 10:341,276:419,193 29 Na Na
26 Hybrid PKS- NRPS Mycgr3G62978 YES NO NO YES 10:425,702:485,734 20 10:439,622:460,865 6
27 PKS Mycgr3G101493 YES NO YES YES 10:1,274,562:1,480,511 54 10:1,436,111:1,437,155 7
28 NRPS-Like Mycgr3G49555 NO NO NO YES 11:393,046:429,601 12 11:409,058:423,584 5
29 PKS Mycgr3G96592 NO YES NO NO 11:566,694:611,143 14 Na Na
30 NRPS-Like Mycgr3G50095 YES YES NO NO 11:823,532:917,882 30 11:849,800:868,675 7
31 NRPS Mycgr3G96900 YES NO YES YES 12:157,368:195,467 15 Na Na
32 NRPS-Like Mycgr3G77312 NO NO NO NO 12:434,096:471,899 9 Na Na
33 PKS Mycgr3G51018 NO NO NO YES 13:656,107:712,077 17 Na Na
34 Terpene Mycgr3G101921, Mycgr3G12838 YES NO YES NO 13:1,116,277:1,139,970 8 Na Na
  1. AntiSMASH and SMURF analysis pipelines were used to predict secondary metabolite clusters in Z. tritici isolate IP0323, identifying a total of 34 putative loci. Ensembl gene identifiers for predicted key biosynthetic genes identified by these analyses are given. Clusters were numbered in ascending numerical order based on their respective location in the Z. tritici genome. In order to identify genes encoding ABC or MFS transporters at each locus, genes were interrogated based on GO-terms GO:0055052 (ATP-binding cassette transporter complex) and GO:0055085 (transmembrane transporter). Transcription factors were identified by screening genes for GO-terms GO:0003677 (DNA binding) and GO:0003700 (transcription factor activity). Subtelomeric clusters were defined as any loci with predicted genes residing within 300 kb of the chromosome end. Interrogation of each individual cluster suggests the key gene of cluster 13 encodes a ketoacyl synthase domain-containing protein, and consequently is likely involved in fatty acid synthesis. Cluster 2 consists of two transport proteins, and lacks a gene encoding a putative key biosynthetic enzyme