TY - JOUR AU - Moll, Karen M. AU - Zhou, Peng AU - Ramaraj, Thiruvarangan AU - Fajardo, Diego AU - Devitt, Nicholas P. AU - Sadowsky, Michael J. AU - Stupar, Robert M. AU - Tiffin, Peter AU - Miller, Jason R. AU - Young, Nevin D. AU - Silverstein, Kevin A. T. AU - Mudge, Joann PY - 2017 DA - 2017/08/04 TI - Strategies for optimizing BioNano and Dovetail explored through a second reference quality assembly for the legume model, Medicago truncatula JO - BMC Genomics SP - 578 VL - 18 IS - 1 AB - Third generation sequencing technologies, with sequencing reads in the tens- of kilo-bases, facilitate genome assembly by spanning ambiguous regions and improving continuity. This has been critical for plant genomes, which are difficult to assemble due to high repeat content, gene family expansions, segmental and tandem duplications, and polyploidy. Recently, high-throughput mapping and scaffolding strategies have further improved continuity. Together, these long-range technologies enable quality draft assemblies of complex genomes in a cost-effective and timely manner. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-017-3971-4 DO - 10.1186/s12864-017-3971-4 ID - Moll2017 ER -