Fig. 6
RT-qPCR validation of selected ten Cd transporter-related DEGs under the control and Cd-treated in roots of two pak choi cultivars. The relative expression levels of each selected gene was determined by 2-ΔΔCT. Each bar represents the mean ± STD of triplicate assays. Values with different letters indicate significant differences at P < 0.05 according to Duncan’s multiple range tests. The ten DEGs including zinc-regulated transporter 2/3 (ZIP2/3), iron-regulated transporter 1 (IRT1), copper transporter 5 (COPT5), multidrug resistance protein 7 (MRP7), metal tolerance protein 3 (MTP3), cation exchanger 4 (CAX4) and cadmium/zinc/copper-transporting ATPase HMA 2/3/4 (HMA2/3/4), were analyzed by RT-qPCR (a-j) and comparized with expression profile by RNA-seq (k)