BMC Genomics

Table 2 Descriptive statistics and expression level of reference genes in (a) ovary and (b) testis as obtained by BestKeeper

From: Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress

a. Ovary
	18S rRNA	rbpl13	gapdh	ß-actin	tubulin	tbp	b2mg	ubi	eef1	g6pd
n	81	81	81	81	81	81	81	81	81	81
geo Mean [CP]	13.62	20.75	35.1	13.79	16.78	33.92	21.7	20.03	20.45	18.91
AR Mean [CP]	13.73	21.08	35.2	14.11	16.79	33.94	21.7	20.89	20.45	18.95
min [Cq]	11.09	16.14	31.34	10.09	16.12	32.89	21.08	14.55	20.28	17.14
max [Cq]	15.88	25.52	37.67	16.67	17.49	35.61	22.51	29.42	20.68	20.18
Std. dev [+/− Cq]	1.58	3.09	2.46	2.66	0.42	0.98	0.51	5.62	0.15	1.14
CV [% Cq]	11.5	14.68	6.99	18.86	2.49	2.9	2.33	26.89	0.75	6.03
b. Testis
	18S rRNA	rbpl13	gapdh	ß-actin	tubulin	tbp	b2mg	ubi	eef1	g6pd
n	81	81	81	81	81	81	81	81	81	81
geo Mean [Cq]	19.83	18.7	33.08	13.49	25.43	27.6	25.76	33.88	22.53	37.59
AR Mean [Cq]	20.19	18.99	33.21	13.76	25.65	27.61	25.76	34.38	22.58	37.61
min [Cq]	15.2	15.32	30.17	10.09	17.39	26.71	24.69	26.26	20.12	35.12
max [Cq]	24.94	24	53.47	16.86	27.65	28.98	27.32	38.94	24.01	39.43
Std. dev [+/− Cq]	3.18	3.06	2.18	2.34	1.96	0.7	0.4	5.25	1.39	1.33
CV [% Cq]	15.74	16.14	6.55	16.98	7.64	2.53	1.56	15.27	6.14	3.53

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