Fig. 2

Proteomic analyses of the HBV mouse model. a Two-dimensional gel electrophoresis analysis of the liver tissue in HBV (+) mice and HBV (−) mice. The liver tissues of HBV (+) and HBV (−) mice were collected at 1, 3 and 6-month post-injection. Total proteins were extracted, and 300 μg protein was analyzed by two-dimensional gel electrophoresis (pH 3–10 NL). The representative pictures of 2-D gel images are shown: the left panels are the 2-D gels of HBV (−) mice, and the right panels are the 2-D gels of HBV (+) mice. Arrows represent the down-regulated proteins, and the circle represents the up-regulated proteins compared with the HBV (−) mice. The experiments were performed in triplicate. b GO analysis of the differently expressed proteins. The chart shows an overview of the gene ontology analysis with up to 10 significantly enriched terms in the BP, CC, and MF categories. The cut-off of P-value is set to 0.05. Terms of the same category are ordered by P-values. Left terms are more significant than the right. Information on the percentage and number of involved proteins in a term is shown on the y-axis. c KEGG Pathway analysis of the differentially expressed proteins. The chart shows the top 10 enriched KEGG Pathways. P value =0.01 and P value =0.05 as two selected cutoffs are highlighted on the figure