Genome-wide gene expression analysis in the placenta from fetus with trisomy 21

BMC Genomics

Table 3 Quantitative real-time PCR results for selected genes

Gene symbol	Chromosome number	Expression change (fold)		P value
Gene symbol	Chromosome number	Microarray	qPCR	P value
Up-regulated genes in trisomy 21
ANKRD37	4	2.064	4.312	0.036
FLT1	13	2.272	5.599	0.002
HUNK	21	2.041	2.189	0.013
IGSF5	21	2.310	2.014	0.017
LEP	7	5.840	2.867	0.024
MCOLN3	1	2.220	7.137	0.006
METTL21C	13	2.905	4.585	0.010
MPZL3	11	2.243	8.906	0.023
SON	21	1.970	4.480	0.032
SYNJ1	21	1.984	5.653	0.025
Down-regulated genes in trisomy 21
COL4A5	X	2.465	5.672	0.003
GJC1	17	2.188	3.714	0.019
GOLIM4	3	2.335	2.857	0.005
HGF	7	2.242	2.787	0.013
MRPL43	10	2.611	2.188	0.015
SUSD2	22	2.324	4.908	0.001
Non-significant genes
PLEK	2	0.874	0.757	0.273
MAPKAP1	9	0.970	0.784	0.499
ATP6	11	1.075	0.838	0.613

Data were normalized to the GAPDH housekeeping gene. Values for microarray data are fold intensity. For each quantitative PCR experiment, values were determined by measuring samples in triplicate (mean ± standard deviation). Each experiment was performed independently at least three times. P values are derived from quantitative real-time PCR data

ISSN: 1471-2164