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Table 3 Quantitative real-time PCR results for selected genes

From: Genome-wide gene expression analysis in the placenta from fetus with trisomy 21

Gene symbol Chromosome number Expression change (fold) P value
Microarray qPCR
Up-regulated genes in trisomy 21
ANKRD37 4 2.064 4.312 0.036
FLT1 13 2.272 5.599 0.002
HUNK 21 2.041 2.189 0.013
IGSF5 21 2.310 2.014 0.017
LEP 7 5.840 2.867 0.024
MCOLN3 1 2.220 7.137 0.006
METTL21C 13 2.905 4.585 0.010
MPZL3 11 2.243 8.906 0.023
SON 21 1.970 4.480 0.032
SYNJ1 21 1.984 5.653 0.025
Down-regulated genes in trisomy 21
COL4A5 X 2.465 5.672 0.003
GJC1 17 2.188 3.714 0.019
GOLIM4 3 2.335 2.857 0.005
HGF 7 2.242 2.787 0.013
MRPL43 10 2.611 2.188 0.015
SUSD2 22 2.324 4.908 0.001
Non-significant genes
PLEK 2 0.874 0.757 0.273
MAPKAP1 9 0.970 0.784 0.499
ATP6 11 1.075 0.838 0.613
  1. Data were normalized to the GAPDH housekeeping gene. Values for microarray data are fold intensity. For each quantitative PCR experiment, values were determined by measuring samples in triplicate (mean ± standard deviation). Each experiment was performed independently at least three times. P values are derived from quantitative real-time PCR data