Integrated analysis of mRNA-seq and miRNA-seq reveals the potential roles of sex-biased miRNA-mRNA pairs in gonad tissue of dark sleeper (Odontobutis potamophila)

Table 1 Relative miRNA expression of top 16 DE miRNAs for comparison of the OT versus OO groups with respect to miRNA-seq and quantitative real-time PCR

miR_name	miR_seq	IlluminamiRNA-seq (log2 fold change)	Regulation (OT vs. OO)	Real-time PCR (log2 fold change)
dre-miR-221-5p_R-4	ACCTGGCATACAATGTAGATTT	−1.74	down	0.25
cgr-miR-221-5p_R-4	ACCTGGCATACAATGTAGATTT	−1.74	down	0.25
aca-miR-138-5p_R + 1	AGCTGGTGTTGTGAATCAGGCCG	2.29	up	2.91
cfa-miR-153_R + 2	TTGCATAGTCACAAAAGTGATC	−0.97	down	−0.36
dre-miR-145-3p	GGATTCCTGGAAATACTGTTCT	1.74	up	1.52
xtr-miR-145_R-2	GTCCAGTTTTCCCAGGAATCCC	2.91	up	2.47
fru-miR-222_R-1	AGCTACATCTGGCTACTGGGTCT	−1.53	down	−1.31
ssa-miR-222a-3p_R-1	AGCTACATCTGGCTACTGGGTCT	−1.53	down	−1.31
ola-miR-139_R + 2	TCTACAGTGCATGTGTCTCCAGT	−1.30	down	−0.87
tgu-miR-139-5p	TCTACAGTGCATGTGTCTCCAGT	−1.30	down	−0.87
dre-miR-143_R-3_1ss18GA	TGAGATGAAGCACTGTAA	2.85	up	2.50
dre-miR-17a-2-3p_L-1_1ss19CA	CTGCAGTGGAGGCACTTAAAGC	−1.07	down	−2.17
dre-miR-222a-5p_R-1_1ss11TG	TGCTCAGTAGGCAGTGTAGATC	−1.43	down	−1.26
ssa-miR-222a-5p_R-2	TGCTCAGTAGGCAGTGTAGATC	−1.43	down	−1.26
dre-miR-133a-5p	AGCTGGTAAAATGGAACCAAAT	4.90	up	5.15
ola-miR-133-5p_R-1	AGCTGGTAAAATGGAACCAAAT	4.90	up	5.15

ISSN: 1471-2164